Patch-clamp fluorometry–based channel counting to determine HCN channel conductance
نویسندگان
چکیده
منابع مشابه
Patch-clamp fluorometry–based channel counting to determine HCN channel conductance
Counting ion channels on cell membranes is of fundamental importance for the study of channel biophysics. Channel counting has thus far been tackled by classical approaches, such as radioactive labeling of ion channels with blockers, gating current measurements, and nonstationary noise analysis. Here, we develop a counting method based on patch-clamp fluorometry (PCF), which enables simultaneou...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 2016
ISSN: 0022-1295,1540-7748
DOI: 10.1085/jgp.201511559